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ab13847). The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/ mL,

ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Antibody Information. Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3, anti- active Caspase 3. Clonality: polyclonal antibody. Comments: validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunocytochemistry; Immunohistochemistry; Western Blot; Flow Cytometry, The following primary antibodies were used: Anti-vimentin (cat.

active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 and rabbit anti-caspase-3 (cat. no. ab13847; 1:1,000; Abcam). The blots were then washed with TBST and incubated with the secondary antibody (cat. no.-2306; cat. ZB no.

2021-02-10 · (PCNA, AB13847, Abcam, MA) or an IgG that recognizes cleaved caspase-3 (1:250, AB13847, Abcam, MA), respectively. Rat anti-mouse CD3 IgG3к (1:20, 550295, BD Pharmingen, CA) was used to visualize naïve T lymphocytes; and polyclonal rabbit anti-CD68 IgG (1:250, AB125212, Abcam, MA) was used to recognize local monocytes and macrophages.

FGL1 expression in the normal human bronchial epithelial cell line Beas-2B, as well as four lung tumor cell lines 2020-05-24 · Background As a nucleolar protein associated with ribosome biogenesis, pescadillo homolog 1 (PES1) has been reported to participate in the development of many cancers. However, its role in prostate cancer is not clearly defined. Therefore, the aim of this study is to explore the effects and the specific mechanism of PES1 in prostate cancer.

active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973

After washing with PBS with 1% Tween-20, the membrane was incubated with respective and species-speciﬁc Horse Radish Peroxidase-labeled secondary antibody Anti-active Caspase 3 antibody (ab13847) Host Organism Rabbit Immunogen Organism Human Isotype.

Consequently, attenuating inflammation by reducing local macrophage accumulation is an appealing approach. This can potentially be accomplished by either blocking blood monocyte recruitment to the plaque or increasing macrophage apoptosis and emigration. Digital data. Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data. I 've been having some issues with detecting cleaved caspase 3, 7 and 9 in my w.blots.
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The intensity of the bands was normalized to the intensity of a reporter protein (actin) with … For immunohistochemistry apoptosis and DNA damage, quantitation was done using antibodies against cleaved caspase 3 (Abcam, ab13847) or γH2AX (clone JBW301; Millipore), respectively. For Western blotting, antibodies against PAXX (Abcam, ab126353) and β-actin (Sigma, A5316) were used. (cat.

You are probably talking about ab13847, not ab31847 (which is used for p72). In my apoptosis studies, I used an antibody from Cell Signaling (#14220), which always worked Caspase-3 in Jurkat Human Cell Line.
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2018-11-14

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application. I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information. Universität Heidelberg.